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Antibacterial Effects of Streptococcus Salivarius K12 on Oral Bacteria
Int J Clin Prev Dent 2016;12(4):209-220
Published online December 30, 2016;  https://doi.org/10.15236/ijcpd.2016.12.4.209
© 2016 Korean Academy of Preventive Dentistry.

Yun-Mi Moon1, Jung-Eun Moon2, Mi-Ra Lee3, Ja-Won Cho4

1Department of Dental Hygiene, Seonam University, Asan, 2Department of Oral Health, Graduate School, Dankook University, 3Department of Dental Hygiene, Baekseok University, 4Department of Preventive Dentistry, College of Dentistry, Dankook University, Cheonan, Korea
Correspondence to: Ja-Won Cho
Department of Preventive Dentistry, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan 31116, Korea. Tel: +82-41-550-1953, Fax: +82-41-553-6582, E-mail: priscus@dku.edu
Received August 16, 2016; Revised December 20, 2016; Accepted December 20, 2016.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Objective: For the purpose of this study is to study the antibacterial effects of probiotics S. salivarius K12 on oral bacteria (Aggregatibacter actinomycetemcomitans, Actinomyces naeslundii, Enterococcus faecalis, Fusobacterium nucleatum, Porphyromonas endodontalis, Porphyromonas gingivalis, Streptococcus gordonii, Streptococcus mutans).Methods: The antimicrobial activity of eight oral bacteria and S. salivarius K12 tests were performed. S. salivarius K12 culture medium and A. actinomycetemcomitans, A. naeslundii, E. feacalis, F. nucleatum, P. endodontalis, P. gingivalis, S. gordonii S. mutans culture alone turbid liquid and mixed, and then the incubator at 37oC in anaerobic conditions and cultured 36 hours. The oral bacteria and S. salivarius K12 using a phase contrast microscope, and the state was observed with bacterial contamination, using a spectrophotometer was an antibacterial test.Results: S. salivarius K12 showed antibacterial effects proportional to the injection amount increase for above certain concentrations on Actinobacillus atinomycetemcomitans, Actinomyces naeslundii, Enterococcus faecalis, pophyromonas gingivalis, Streptococcus Streptococcus mutans. S. salivarius K12 increased the growth of germs in a condition of low concentration of Fusobaterium nucleatum, pophyromonas endodontalis, but showed antibacterial effects proportional to the injection amount increase for above certain concentrations.Conclusion: Based on the experiment results of probiotics S. salivarius K12 on the oral bacteria causing oral cavity, S. salivarius K12 showed antibiosis against oral bacteria when it remains inside the mouth for above certain concentrations. Accordingly, it is thought that it would be effective to use S. salivarius K12 as a clinical method of preventing oral cavity.
Keywords : probiotics, bacteria, streptococcus salivarius
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