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Clinical Efficacy of a New Mouthwash Containing Essential Oil of Cardamom in Reducing Volatile Sulphur Coumpounds Concentration
Int J Clin Prev Dent 2014;10(4):237-242
Published online December 30, 2014
© 2014 International Journal of Clinical Preventive Dentistry.

Suci Erawati1, Anton Rahardjo2, Sondang Pintauli3

1Doctoral Student, Graduate School of Faculty of Dentistry, University of Sumatera Utara, Medan, 2Department of Preventive Dentistry, Faculty of Dentistry, University of Indonesia, Jakarta, 3Department of Preventive Dentistry, Faculty of Dentistry, University of Sumatera Utara, Medan, Indonesia
Correspondence to: Anton Rahardjo
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective: The aim of this study was to assess the clinical efficacy of new mouthwash based on kapulaga fruit (Amomum cardamommum L.) containing 0.5% essential oil and compared to 0.5% Listerine? as a positive control and placebo in reducing volatile sulphur compounds (VSCs) concentration of halitosis subjects. Methods: A randomized, double blinded, cross-over design was conducted among 20 healthy volunteers in Medan, Indonesia. Subjects were divided into 3 groups (Cardamom, Listerine?, placebo group) and instructed to rinse 10 ml of experiment
and control mouthwash twice per day for 5 days. After one week washout periods, each group used the opposite mouthwash. The concentration of VSCs, hydrogen sulfide, methyl mercaptan, and dimethyl sulfide were assessed twice daily for 5 days (morning at 5 a.m. and afternoon at 11 a.m.) by using oral chroma and organoleptic measurement. The three mouthwash were analyzed statistically by repeated measures ANOVA. Results: Either Cardamom or Listerine? was effective in reducing VSCs for up to 4 to 5 hours after mouth rinsing, while placebo not. An analysis showed significant differences of cardamom essential oil in mouthwash and positive control group
(p<0.05) from 1st and 5th day while no significant difference in placebo group (p>0.05). Results showed that Cardamom more effective than the other two mouthwashes. Unlike other mouthwash in general, the advantage of cardamom essential oil in mouthwash is alcohol-free, that could avoid from oral pain and sensitivity. Conclusion: It can be concluded that 0.5% cardamom containing essential oil in mouthwash is useful to overcome halitosis.
Keywords : essential oils, volatile oils, halitosis
  1. Scully C, Greenman J. Halitosis (breath odor). Periodontol 2000 2008;48:66-75.
    Pubmed CrossRef
  2. R?sing CK, Jonski G, Rølla G. Comparative analysis of some mouthrinses on the production of volatile sulfur-containing compounds. Acta Odontol Scand 2002;60:10-2.
    Pubmed CrossRef
  3. Rayman S, Almas K. Halitosis among racially diverse populations:an update. Int J Dent Hyg 2008;6:2-7.
    Pubmed CrossRef
  4. R?sing CK, Loesche W. Halitosis: an overview of epidemiology, etiology and clinical management. Braz Oral Res 2011;25:466-71.
    Pubmed CrossRef
  5. Yaegaki K, Coil JM. Examination, classification, and treatment of halitosis; clinical perspectives. J Can Dent Assoc 2000;66:257-61.
  6. Murata T, Rahardjo A, Fujiyama Y, Yamaga T, Hanada M, Yaegaki K, et al. Development of a compact and simple gas chromatography for oral malodor measurement. J Periodontol 2006;77:1142-7.
    Pubmed CrossRef
  7. Lesmana S, Darwita RR, Rahardjo A. Efficacy of mouthwash containing curcuma xanthorrhiza on lactoperoxidase system activity and halitosis risk. Int J Clin Prev Dent 2013;9:207-10.
  8. Tsai CC, Chou HH, Wu TL, Yang YH, Ho KY, Wu YM, et al. The levels of volatile sulfur compounds in mouth air from patients with chronic periodontitis. J Periodontal Res 2008;43:186-93.
    Pubmed CrossRef
  9. Rosenberg M, Gelernter I, Barki M, Bar-Ness R. Day-long reduction of oral malodor by a two-phase oil:water mouthrinse as compared to chlorhexidine and placebo rinses. J Periodontol 1992;63:39-43.
    Pubmed CrossRef
  10. Loesche WJ, Kazor C. Microbiology and treatment of halitosis. Periodontol 2000 2002;2:256-79.
  11. Shinada K, Ueno M, Konishi C, Takehara S, Yokoyama S, Kawaguchi Y. A randomized double blind crossover placebo-controlled clinical trial to assess the effects of a mouthwash containing chlorine dioxide on oral malodor. Trials 2008;9:71.
    Pubmed CrossRef
  12. Anyanwu OC, Baugh KK, Bennett SB, Johnson JM, Madlock RL, Pollard NE, et al. Comparison of the antibacterial effectiveness of alcohol-containing and non-alcohol-containing mouthwashes. J Sci 2011;2:7-12.
  13. Kaushik P, Goyal P, Chauhan A, Chauhan G. In vitro evaluation of antibacterial potential of dry fruit extracts of elettaria cardamomum maton (chhoti elaichi). Iran J Pharm Res 2010;9:287-92.
  14. Arora DS, Kaur GJ. Antibacterial activity of some Indian medicinal plants. J Nat Med 2007;61:313-7.
  15. Malhotra S, Yeltiwar RK. Evaluation of two mouth rinses in reduction of oral malodor using a spectrophotometric technique. J Indian Soc Periodontol 2011;15:250-4.
    Pubmed CrossRef
  16. Ministry of Health, Republic of Indonesia. National policy on traditional medicines 2007 [Internet]. Jakarta: Ministry of Health, Republic of Indonesia [cited 2014 Sep 27].
  17. Tonzetich J. Oral malodour: an indicator of health status and oral cleanliness. Int Dent J 1978;28:309-19.

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