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A study to comparison of characterizations of Zinc Oxide Eugenol based and Mineral Trioxide Aggregate based root canal sealers
Int J Clin Prev Dent 2021;17(3):152-152
Published online September 30, 2021;
© 2021 International Journal of Clinical Preventive Dentistry.

Seok-Eun Lee1, Ja-Won Cho1, Hyun-Jun Yoo1, Myung-Gu Lee2, Yeol-Mae Jeon1, Da-Hui Kim3, Hye-Won Park1

1Department of Preventive Dentistry, College of Dentistry, Dankook University, Cheonan, Republic of Korea
2College of Biomedical & Health Science, Department of Biomedical Chemistry, Konkuk University, Chungju, Republic of Korea
3Department of Dental Hygiene, College of Health Science, Dankook University, Cheonan, Republic of Korea
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective: The aims of this study were to investigate antimicrobial activity and cytotoxicity of zinc oxide eugenol (ZOE) based and mineral trioxide aggregate (MTA) based root canal seal.
Methods: The root canal sealers in this study are Tubli-Seal as a ZOE based sealer and ProRoot as a MTA based sealer. The antimicrobial activity of ProRoot and Tubli-seal was carried out by susceptibility assay using Enterococcus faecalis, Porphyromonas endodontalis, and Porphyromonas gingivalis. The cytotoxicity of the root canal sealers was investigated by MTT assay, real-time RT-PCR, and ELISA after treatment of the sealers onto pulp cells. Also, the components of the root canal sealers were analyzed by X-ray fluorescence analysis.
Results: MTA based sealer showed stronger antimicrobial activity against E. faecalis than ZOE based sealer, and the antimicrobial activity of the both sealers against P. endodontalis and P. gingivalis showed no significant difference. ZOE based sealer showed strong cytotoxicity for pulp cells and induced expression and production of inflammatory cytokines. However, MTA based sealer did not affect cell viability of pulp cell and did not significantly induce expression of inflammatory cytokines such as TNF-α, IL-6 and IL-8. In analysis of the components, MTA bases sealer has more kinds of metal oxides than ZOE based sealer.
Conclusion: MTA based sealer had stronger antimicrobial activity against E. faecalis compared to ZOE based sealer and did not significantly show cytotoxicity to pulp cells. In the basis of these results, MTA based sealer may be a suitable material to root canal therapy.
Keywords : Words: zinc oxide eugenol, mineral trioxide aggregate, root canal sealer

September 2021, 17 (3)