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Inhibitory Effect of EUTHYMOL Original Toothpaste on VSC-producing Oral Bacteria
Int J Clin Prev Dent 2021;17(2):75-75
Published online June 30, 2021;
© 2021 International Journal of Clinical Preventive Dentistry.

Hyojin Kim1, Wonkyung Choi2, Wonkyung Lee2, Wonchan Kim2, Kyounghee Oh1, Wonho Ha3

1Preventive Dentistry Research Team, LG H&H Research Center, Seoul Korea, 2Deodorizing Tech Project, LG H&H Research Center, Daejeon, Korea, 3Oral Health R&D Division, LG H&H Research Center, Seoul, Korea
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective: Oral malodor (bad breath) is generally the result of microbial metabolism, and volatile sulfur compounds (VSC) producing bacteria are mainly present in biofilm of tongue dorsum, are the main cause of halitosis. Many studies have revealed that these bacteria are also associated with periodontal disease. We developed a flavoring agent by combining ingredients that identified to have oral antibacterial properties, and applied to EUTHYMOL original toothpaste. In this study, we examine the effect of suppressing oral bacteria and reducing VSC production of the toothpaste.
Methods: 1) Oral bacteria inhibitory test: P. gingivalis, F. periodonticum are cultured in BHI medium at anaerobic incubation system. The toothpaste samples were treated for 24h on bacterium culture condition, VSC was detected by Oral Chroma. 2) Salivary incubation test: Saliva suspension from healthy adults was incubated anaerobically with amino acids (cysteine and methionine) and toothpaste samples for 4h, and VSC was measured by Oral Chroma.
Results: As a result of the VSC inhibition test of oral bacteria, 82.6% of P. gingivalis inhibition rate was revealed compared to the non-treated group, and 1.41-fold difference compared to the unscented toothpaste treatment group. Also, 49.2% of F. periodonticum inhibition rate was showed compared to the non-treated group. In saliva incubation test, the VSC inhibition rate was 58.9% compared to the non-treated group, and 1.45-fold difference compared to the unscented toothpaste treatment group.
Conclusion: We revealed the anti-microbial effect of EUTHYMOL original toothpaste on halitosis-inducing oral bacteria. Through this study, the effect of suppressing bad breath through VSC inhibition in oral cavity can be expected.
Keywords : halitosis, VSC, bacteria, saliva, EUTHYMOL

June 2021, 17 (2)