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Prevalence of Periodontopathogens Using Real-Time Polymerase Chain Reaction in Adolescents
Int J Clin Prev Dent 2019;15(4):220-225
Published online December 31, 2019;
© 2019 International Journal of Clinical Preventive Dentistry.

Eun-Ji Lee1, Jung-Eun Park2, Hyun-Jun Yoo3, Ja-Won Cho1,2,3

Departments of 1Oral Health and 2Dental Hygiene, College of Health Science, Dankook University, 3Department of Preventive Dentistry, College of Dentistry, Dankook University, Cheonan, Korea
Correspondence to: Ja-Won Cho
*This article is based on Eun-Ji Lee’s graduate thesis.
Received October 23, 2019; Revised November 16, 2019; Accepted November 18, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objectives: The author had conducted this study for the purpose to determine the prevalence of oral microbes known as the cause of periodontopathogens in adolescents in Korea.
Methods: Oral examination was conducted for 146 students. The amount of bacteria associated with periodontal disease such as Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia, and Prevotella nigrescens from subjects’ oral cavity by real-time polymerase chain reaction was counted, and prevalence of periodontopathogens additionally investigated.
Results: The percentages of specific intra-oral microbes found in the subjects were elementary school student P. gingivalis 5%, T. denticola 7.5%, F. nucleatum 100%, P. intermedia 10%, and P. nigrescens 60%, middle school student P. gingivalis 12.2%, T. denticola 0%, F. nucleatum 100%, P. intermedia 12.2%, and P. nigrescens 69.4%, high school student P. gingivalis 3.5%, T. denticola 0%, F. nucleatum 98.2%, P. intermedia 7%, and P. nigrescens 68.4%.
Conclusions: The microbe detected with the largest amount among the oral microbes tested was F. nucleatum, followed by P. nigrescens in the order, therefore the most effective and safe method of preventing periodontal disease is healthy tooth maintenance, oral hygiene and regular oral examinations.
Keywords : microbiology, adolescent, oral health
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December 2019, 15 (4)