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Analysis of Crystal Structure of Bone Graft Material Using Analyses of X-Ray Diffraction and Scanning Electron Microscope Image
Int J Clin Prev Dent 2019;15(4):215-219
Published online December 31, 2019;
© 2019 International Journal of Clinical Preventive Dentistry.

Kiseok Hong

Department of Periodontics, Moon Dental Hospital, Cheonan, Korea
Correspondence to: Kiseok Hong
Received October 23, 2019; Revised December 6, 2019; Accepted December 11, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objectives: The present study was to analyze the crystal structure of Bio-Oss, the representative bone graft material, through X-ray diffraction (XRD) and scanning electron microscope (SEM) image analyses.
Methods: Xenogeneic bone (Bio-Oss; Geistlich Biomaterials, Wolhusen, Switzerland) for bone graft of which particle size was in the range of 0.25-1.0 mm was used for the present study. Specimens were quickly frozen and then pulverized to conduct XRD analysis. A SEM was used to observe materials in forms of powder and monolith, respectively.
Results: The XRD pattern of the specimen showed components of bone and hydroxyapatite (HA). The broad peak appearing in the vicinity of 12.6° was seen as organic material. In the SEM, pores were observed together with the coagulation of small particles. Particles were concluded as those of small HA with sizes of around 50 nm.
Conclusions: Xenogeneic Bio-Oss consist of apatite of low crystallinity similar to the bony tissue of humans. Thus, consequences of bone graft employing Bio-Oss are expected to be different from those of bone graft using bone graft materials of high crystallinity regarding the healing process.
Keywords : apatite, bone, regeneration, hydroxyapatites, implants
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December 2019, 15 (4)