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Changes in Pathogenic Microorganisms in Different Denture Management Methods
Int J Clin Prev Dent 2019;15(3):152-158
Published online September 30, 2019;
© 2019 International Journal of Clinical Preventive Dentistry.

Sun Ae Kim1, Chang Yoon Kim2

1Department of Public Health Studies, Yeungnam University Graduate School, Gyeongsan, 2Department of Preventive Medicine & Public Health, Yeungnam University College of Medicine, Daegu, Korea
Correspondence to: Chang Yoon Kim
Received September 17, 2019; Revised September 23, 2019; Accepted September 23, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective: The control group consisted of 28 subjects applying the conventional denture management method and the experimental group consisted of 28 subjects applying the newly designed denture management method. Saliva was sampled from both the control and experimental groups and then analyzed by real-time polymerase chain reaction to determine the change in the kind and quantity of microorganisms in the oral cavity.

Methods: The interventional group was educated by simulation methods, using educational material on trainings for tooth brushing and denture cleaning. The control group was provided only a brief description of general denture management method.

Results: There was a quantitative change in pathogenic microorganisms over the three months period after the denture implantation. Porphyromonas gingivalis (Pg), Treponema denticola (Td), and Prevotella nigrescens (Pn) were significantly decreased in the interventional group (p<0.05). In comparison, the control group exhibited significant increase in Pg, Tannerella forsythia (Tf), Td, Fusobacterium nucleatum (Fn), Prevotella intermedia (Pi) and Pn (p<0.05). Three months after denture in implantation Pg, Tf, Td, Fn, Pi, Pn, control group was significantly high than interventional group (p<0.05).

Conclusion: The results of this research represent that the denture management method has an important effect on quantitative changes of pathogenic microorganisms in the elderly. Therefore, it is important to inhibit the increase of pathogenic microorganisms by applying the newly designed denture management method. Furthermore, the newly designed denture management method would prevent oral diseases, including periodontal disease, by decreasing pathogenic microorganisms in the oral cavity.
Keywords : denture care, oral hygiene, gene analysis, real-time polymerase chain reaction, microorganism
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September 2019, 15 (3)