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Changes in Oral Microbial Population through Dental Scaling and Dental Polishing
Int J Clin Prev Dent 2019;15(1):62-67
Published online March 30, 2019;  https://doi.org/10.15236/ijcpd.2019.15.1.62
© 2019 International Journal of Clinical Preventive Dentistry.

Jin-Young Lee1, Kyung-Hui Moon2

1Department of Dental Hygiene, Gumi University, Gumi, 2Department of Dental Hygiene, Jinju Health College, Jinju, Korea
Correspondence to: Kyung-Hui Moon, E-mail: next77_kr@naver.com, https://orcid.org/0000-0002-4584-4237
Received February 24, 2019; Revised March 18, 2019; Accepted March 20, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Objective: This study is intended to investigate the effectiveness of scaling and dental polishing and their correlation to oral microorganisms by examining the change in oral microbial population after scaling and dental polishing through clinical tests and real-time polymerase chain reaction.
Methods: This study examined 117 male and female adults of 20 years or older who visited the dental clinics in Daejeon, Korea. It used the real-time PCR to analyze the change of the oral microbial population before and after scaling and dental polishing.
Results: Before scaling, the prevalence rate of Fusobacterium nucleatum was 100.0%, meaning that it was prevalent in all examined subjects, Parvimonas micra 85.5%, Prevotella intermedia 76.1%, and Tannerella forsythia 72.6% while the prevalence rate of P. micra was 75.2% and P. intermedia 72.6% after scaling, and P. micra 45.3% and P. intermedia 66.7% after dental polishing. After scaling, all 11 oral bacterial pathogens including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, T. forsythia, Treponema denticola, P. intermedia, F. nucleatum, P. micra, Campylobacter rectus, Eubacterium nodatum, Prevotella nigrescens, and Eikenella corrodens showed a considerably reduced population and had statistically significant differences (p<0.05). After dental polishing, 10 oral bacterial pathogens except for A. actinomycetemcomitans showed a considerably reduced population and had statistically significant differences (p<0.05).
Conclusion: Considering the results that the considerably reduced prevalence rate of 11 oral pathogenic microbes related to periodontal disease after scaling and dental polishing, we concluded that regular scaling and selective dental polishing are necessary for the periodontal treatment of the adult.
Keywords : dental scaling, dental polishing, periodontal diseases
References
  1. Paik DI, Kim HD, Jin BH, Park YD, Shin SC, Cho JW, et al. Clinical preventive dentistry. 5th ed. Seoul: Komoonsa; 2011:79.
  2. Kim SL, Kwak JS, Kim JG, Kim YH, Kim JH, Lee MH, et al. Oral prophylaxis. Seoul: Deahan Narea Publishing; 2015:21-2.
  3. Cho HB. Multiplex real-time PCR for simultaneous detection of 6 Periodontopathic bacteria. J Microbiol 2013;49:292-6.
    CrossRef
  4. Jo MJ, Lee ES, Bark JR, Jeong JY, Kwon SB, Ham KS, et al. Dental prophylaxis. Seoul: Komoonsa; 2016:161-200.
  5. Peters IR, Helps CR, Hall EJ, Day MJ. Real-time RT-PCR: considerations for efficient and sensitive assay design. J Immunol Methods 2004;286:203-17.
    Pubmed CrossRef
  6. Health Insurance Review & Assessment Service. Medical expenses index 2016 [Internet]. Health Insurance Review & Assessment Service.
  7. Jeong HJ, Kim HJ. Dental oral microorganism changes according to adult oral health knowledge and behavior. J Korean Acad Dent Health 2018;6:24-31.
  8. Loomer PM. Microbiological diagnostic testing in the treatment of periodontal diseases. Periodontol 2000 2004;34:49-56.
    Pubmed CrossRef
  9. Kim YK, Han MD. Oral microbiology. 4th ed. Seoul: Komoonsa; 2010:330.
  10. Dzink JL, Socransky SS, Haffajee AD. The predominant cultivable microbiota of active and inactive lesions of destructive periodontal diseases. J Clin Periodontol 1988;15:316-23.
    Pubmed CrossRef
  11. Kim JG, Chae GK, Cho KS, Moon IS. The effects of scaling on the clinical parameters and subgingival microflora of human periodontal disease. J Korean Acad Periodontol 1990;20:149.
  12. Kim SM, Yang KH, Choi NK, Kang MS, Oh JS. Quantitative detection Of Periodontopathic bacteria using real-time PCR. J Korean Acad Pediatr Dent 2008;35:494-503.
  13. Suzuki N, Yoshida A, Saito T, Kawada M, Nakano Y. Quantitative microbiological study of subgingival plaque by real-time PCR shows correlation between levels of Tannerella forsythensis and Fusobacterium spp. J Clin Microbiol 2004;42:2255-7.
    Pubmed CrossRef
  14. Hong MH. Study on detection of Oral bacteria in the saliva and risk factors of adults. J Korea Acad-Ind Cooper Soc 2014;15:5675-82.
    CrossRef
  15. Johnson WN, Wilson JR. The application of the ultrasonic dental unit to scaling procedures. J Periodontol 1957;28:264-71.
    CrossRef
  16. Lim DS. The morphological observation of the surface roughness of dental hard tissue after root planing, ultrasonic scaling and laser treatment followed by oral prophylaxis. Annu Bull Seoul Health Jr Coll 1997;17:323-33.
  17. Back HJ, Mok SK, Shin HS. Comparison of recolonization of the subgingival microflora after scaling and root planing on single and multiroot periodontal pockets. J Periodontal Implant Sci 1994;24:483-92.
  18. Moon KH, Lee JY, Kang YJ. Study of oral microbial prevalence and oral health in adults. Int J Clin Prev Dent 2018;14:264-70.
    CrossRef
  19. Yun JH, Park JE, Kim DI, Lee SI, Choi SH, Cho KS, et al. Identification of putative periodontal pathogens in Korean chronic periodontitis patients. J Periodontal Implant Sci 2008;38:143-52.
    CrossRef


June 2019, 15 (2)