search for


Antimicrobial Activity of Essential Oil against Oral Strain
Int J Clin Prev Dent 2018;14(4):216-221
Published online December 31, 2018;
© 2018 Korean Academy of Preventive Dentistry.

Chungmu Park1, Hyunseo Yoon2

Departments of 1Clinical Laboratory Science and 2Dental Hygiene, Dong-Eui University, Busan, Korea
Correspondence to: Hyunseo Yoon
Department of Dental Hygiene, Dong-Eui University, 176 Eongwang-ro, Busanjin-gu, Busan 47340, Korea. 
Tel: +82-51-890-2688, Fax: +82-505-182-6878, E-mail:
Received September 6, 2018; Revised November 2, 2018; Accepted December 4, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective: In this study, we tried to investigate the antimicrobial activity of natural herbaceous plant-derived essential oils against oral disease-causing bacterial strains and establish the scientific evidences to apply them to the dentifrice, mouth-wash, and other uses.
Methods: We used the disk diffusion method with 23 types of 100% natural essential oils to verify the antimicrobial effect against Streptococcus mutans, Porphyromonas gingivalis, and Lactobacillus rhamnosus. After adhering to the surface of the plate culture medium, 20 μl of the essential oil was prepared, and the diameter of the transparent ring was measured after incubation for 24 hours. All the experiments were repeated three times.
Results: Among 23 types of oils, 13 were effective against all three strains: myrrh, ginger, basil, carrot seed, tea tree, patchouli, ylang ylang, cypress, lemongrass, cinnamon, peppermint, lavender, and eucalryptus. Seventeen oils were effective against S. mutans, and myrrh, basil, and carrot seed showed high antimicrobial activity. Eighteen oils were effective against P. gingivalis, and tea tree, carrot seed, and cinnamons showed high antimicrobial activity. Sixteen oils were effective against L. rhamnosus, and carrot seed and peppermint cinnamon showed high antimicrobial activity.
Conclusion: Consequently, 13 essential oils showed the antimicrobial activity against three bacterial strains, which indicates these essential oils might be used as the effective materials to suppress the growth of oral-disease inducing microorganisms. Moreover, essential oils that have been analyzed activities in this study will be mixed up within the constant range and analyzed their antimicrobial effects to examine the synergistic activity among them.
Keywords : anti-microbial agents, essential oil, Lactobacillus rhamnosus, Porphyromonas gingivalis, Streptococcus mutans

December 2018, 14 (4)