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Strengthened Antioxidative Potential by Gelidium amansii Ethanol Extract through the Induction of Phase II Enzymes in Human Gingival Fibroblast Cells
Int J Clin Prev Dent 2018;14(3):157-161
Published online September 30, 2018
© 2018 International Journal of Clinical Preventive Dentistry.

Chung-Mu Park1, Hyun-seo Yoon2

Departments of 1Clinical Laboratory Science and 2Dental Hygiene, Dong-Eui University, Busan, Korea
Correspondence to: Hyun-seo Yoon Department of Dental Hygiene, Dong-Eui University, 176 Eomgwang-ro, Busanjin-gu, Busan 47340, Korea. Tel: +82-51-890-2688, Fax: +82-505-182-6878, E-mail: yoonhs@deu.ac.kr https://orcid.org/0000-0002-7455-5506
Received August 27, 2018; Revised September 5, 2018; Accepted September 17, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Objective: Gelidium amansii is one sort of edible red alga distributed along the coast of Northeast Asian countries. G. amansii has been consumed as an important food source as well as a traditional medicine which was known for its regulatory effects against blood pressure and blood glucose. In this study, antioxidative activity of G. amansii ethanol extract (GAEE) was investigated through analysis of reactive oxygen species (ROS) formation and the induction of antioxidative enzymes in human gingival fibroblast-1 (HGF-1) cells. 
Methods: ROS scavenging assay and cell viability were used to analyze the antioxidative activity of GAEE in HGF-1 cells. In order to estimate the expression of antioxidative enzymes, Western blot analysis was applied.
Results: GAEE significantly scavenged lipopolysaccharide isolated from Porphyromonas gingivalis stimulated intracellular ROS production in a dose-dependent manner without any cytotoxicity. To estimate the antioxidative capacity of GAEE as a potent ROS scavenger, tert-butyl hydroperoxide was applied to induce oxidative initiated cell damage in HGF-1 cells and cell viability was also significantly attenuated by GAEE treatment. Therefore, heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO1), have known for their potent antioxidative activity, were analyzed whether they were induced by GAEE treatment or not. Both antioxidative enzymes were also significantly induced by GAEE treatment. In addition, GAEE treatment stimulated the activation of the transcription factor for antioxidative enzymes, nuclear factor erythroid 2-related factor 2 (Nrf2).
Conclusion: Consequently, GAEE strengthened HO-1 and NQO1 mediated antioxidative potential through the regulation of Nrf2 pathway in HGF-1 cells.
Keywords : Gelidium amansii, heme oxygenase-1, NAD(P)H:quinone oxidoreductase 1, NF-E2-related factor 2


September 2018, 14 (3)