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A Clinical Study on the Decreasing Rate of Caries Related Oral Micro-Organisms by a Time of Tooth-Brushing with Sodium Fluoride Contained Dentifrice
Int J Clin Prev Dent 2018;14(2):140-144
Published online June 30, 2018;
© 2018 International Journal of Clinical Preventive Dentistry.

Hee Ja Na1, Ji-Yeon Kwak2

1Department of Dental Hygiene, Honam University, Gwangju, 2Department of Medical Welfare, Kyungbok University, Namyangju, Korea
Correspondence to: Hee Ja Na
Department of Dental Hygiene, Honam University, 417 Eodeung-daero, Gwangsan-gu, Gwangju 62399, Korea. 
Tel: +82-62-940-3821, Fax: +82-62-940-3821, E-mail: artnahe@
Received May 9, 2018; Revised June 11, 2018; Accepted June 13, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective: In order to know how much decrease the oral micro-organisms related with the dental caries by a time of tooth-brushing, a clinical study was performed. 
Methods: Thirty adults age 30 to 60 years of the volunteers with compensation were collected and checked such examination as modified caries activity test with modified Snyder test, the amounts of cocci typed oral micro-organism examined by use of the phase contrast microscope and real time polymerase chain reaction test with sending the gaggled samples to professional institute of oral micro-organisms with genetic analysis method.
Results: A total of 71.9% of decreasing rate of caries activity on Snyder test and 58.5% of decreasing rate on the amounts of cocci typed oral micro-organism by a time of tooth-brushing with the experimental dentifrice. 32.9% and 42.2% of decreasing rate of the amounts of Streptococcus mitis and S. mutans after a time of tooth-brushing with the experimental dentifrice. 
Conclusion: A time of tooth-brushing could be diminished the caries related oral micro-organisms as 30% to 40% and the information could be used for tooth-brushing instruction.
Keywords : oral micro-organism, tooth-brushing, fluoride dentifrice
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