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Reliability Assessment of Multiplex Reverse Transcription- Polymerase Chain Reaction Method for Dental Caries Bacteria
Int J Clin Prev Dent 2017;13(3):135-140
Published online September 30, 2017;  https://doi.org/10.15236/ijcpd.2017.13.3.135
© 2017 International Journal of Clinical Preventive Dentistry.

Seong-Kug Jeong1, Joo-Young Lee2, Kyung-Sook Hwang3, Yong-Duk Park1, Ja-Won Cho4

1Department of Preventive Dentistry, School of Dentistry, Chosun University, Gwangju, 2Department of Dental Hygiene, Gangdong University, Eumseong, 3Department of Dental Technology, Shinhan University, Uijeongbu, 4Department of Preventive Dentistry, College of Dentistry, Dankook University, Cheonan, Korea
Correspondence to: Ja-Won Cho
Department of Preventive Dentistry, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan31116, Korea. Tel: +82-41-550-1953, Fax: +82-41-553-6582, E-mail: priscus@dku.edu
Received July 13, 2017; Revised September 4, 2017; Accepted September 6, 2017.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Objective: The purpose of this study is to confirm the usefulness of RT-PCR method for the prevention of dental caries by quantitative analysis of dental caries causing dental caries, and to widely used instruments for screening oral disease bacteria.Methods: We selected 68 patients between the ages of 7 and 60 years who came to the dental clinic for preventive dentistry. Patients with systemic disease, antibiotics within the last month, dentures, and pregnant women were excluded from the study. Patients who participated in the experiment were slaughtered for 30 seconds with a squeeze solution (12 ml), and then spit into a 50 ml conical tube. The procedure for collecting DNA from saliva is as follows.Results: Streptococcus mitis, Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus casei were tested to determine the difference between SRT-PCR and MRT-PCR when measuring the specificity and sensitivity of primers and probes containing specific nucleotide sequences of bacteria. Four kinds of bacteria were combined and analyzed by MRT-PCR to compare Ct values. When comparing the mean and standard deviation of the Ct values of SRT-PCR and MRT-PCR, it was found that there was no statistically significant difference between the two groups.Conclusion: MRT-PCR showed that the accuracy of quantitative analysis was the same as that of SRT-PCR, and there was no statistically significant difference. Therefore, it is expected that it will be a very useful quantitative method for screening the causative organisms of dental caries or checking the treatment results in the dental clinic.
Keywords : bacterial load, dental caries, polymerase chain reaction
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September 2017, 13 (3)