Fig. 5. Effect of toothpaste including rosemary extract on early biofilm of S. mutans. Early biofilm of S. mutans using the mixture of CCM and TSB was formed in the presence or the absence of toothpaste including rosemary extract at concentration of 125 mg/ml. The biofilm was stained with live/dead bacterial staining kit, and then analyzed with CLSM. The image was analyzed with ZEN software. Green and red color indicate live and dead bacteria in the biofilm, respectively.
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